PlasmidSeq is Cyclomics' advanced solution for sequencing entire plasmids with single-molecule resolution. As plasmids are the natural substrate for Rolling Circle Amplification (RCA), we leverage our deep expertise in RCA and consensus generation to deliver high-quality, end-to-end plasmid sequencing. This technology provides unparalleled accuracy, ensuring comprehensive plasmid analysis from start to finish. Whether you need to verify plasmid constructs, analyze complex plasmid libraries, or sequence unknown vectors, PlasmidSeq delivers precise and reliable results tailored to your needs.

Key Applications:

Quality Control of Plasmid Libraries:PlasmidSeq is perfect for quality control (QC) analysis of large plasmid libraries, where high accuracy is essential to ensure that each plasmid construct is correctly assembled and free from errors. Researchers working on gene therapies, molecular cloning, or protein expression can rely on PlasmidSeq for precise verification of their plasmid constructs.

Monoclonal Verification: In the development of monoclonal plasmid clones, precise sequencing is critical to ensure that the desired insert and vector backbone are correctly assembled without mutations. PlasmidSeq provides high-resolution sequencing for this purpose, delivering reliable data to confirm the accuracy of your monoclonal plasmid constructs.

De-Novo Sequencing of Unknown Vectors:PlasmidSeq excels in de-novo sequencing, making it an ideal tool for identifying unknown or novel plasmid vectors. Whether you're working with uncharacterized plasmids from bacterial samples or creating custom vectors for synthetic biology, PlasmidSeq can provide a complete and detailed sequence of the plasmid, even if its structure is unknown.

Mutation Analysis in Engineered Vectors: For researchers engineering plasmids for CRISPR, gene editing, or synthetic biology applications, ensuring that the plasmid sequences are accurate and free from unintended mutations is crucial. PlasmidSeq allows for the in-depth analysis of engineered vectors to identify point mutations, insertions, deletions, or rearrangements, giving you confidence in your constructs.