Question 1

Which tumour types can Epinn classify?

Accepted Answer

Epinn currently supports CNS tumour and leukemia classification. For CNS tumours, Epinn includes the Sturgeon and Argus-CNS models. For leukemia, Epinn includes the Lamprey and Marlin models. New tumour classification models are under development.

Question 2

Is Epinn free or open-source tumour classification software?

Accepted Answer

No. Epinn is a premium tumour classification software solution designed for rapid, reliable analysis of methylation sequencing data. Epinn is not open-source or freely available, allowing us to continuously develop and maintain the software and its tumour classification models.

Question 3

What data does Epinn need to classify tumours?

Accepted Answer

Epinn requires DNA methylation sequencing data to perform tumour classification. The software analyses methylation data to classify tumours using its machine-learning models.

Question 4

Which sequencing platforms and data formats does Epinn support?

Accepted Answer

Epinn currently supports Oxford Nanopore Technologies (ONT) sequencing data and Illumina IDAT/EPIC Array data. This allows Epinn to be used with both nanopore sequencing and Illumina methylation array workflows.

Question 5

Do I need to preprocess my data before using Epinn?

Accepted Answer

The required preprocessing depends on the sequencing platform.
ONT: Modified-base calling and alignment using MinKNOW are required. No additional preprocessing is needed before running Epinn.
Illumina: Standard processing settings are sufficient for Epinn.

Question 6

What are the system requirements of Epinn?

Accepted Answer

Epinn can run on a standard high-performance workstation. The minimum and recommended specifications are:Minimum specifications (not recommended for real-time sequencing):8-core CPU16 GB RAM200 GB SSD storage
Recommended specifications:10-core CPU64 GB RAM300 GB SSD storage
For real-time tumour classification during sequencing, we recommend using the recommended system configuration.

Question 7

Does Epinn require other software or dependencies?

Accepted Answer

No. Epinn is a standalone tumour classification software solution and can be installed without additional software dependencies.

Question 8

Do I need bioinformatics expertise to install or use Epinn?

Accepted Answer

No. Epinn has been designed as a plug-and-play tumour classification solution. Installation and operation do not require specialist bioinformatics knowledge, making Epinn accessible to users without extensive bioinformatics expertise.

Question 9

Do I need command-line knowledge to use Epinn?

Accepted Answer

No. Epinn does not require command-line expertise. The software is designed with a user-friendly graphical interface, allowing users to perform the required actions using a mouse rather than command-line tools.

Question 10

Does Epinn offer a free trial?

Accepted Answer

Yes. A 1-3 month free trial can be made available for technical validation within your organisation. This allows users to evaluate Epinn with their own data and assess its integration into their existing workflow.

Question 11

What sample purity is required for Epinn?

Accepted Answer

The required sample purity depends on the tumour classification model being used. In general, Epinn is designed for tumour biopsy samples. For the leukemia classification models, peripheral blood and bone marrow samples can also be used.For specific sample requirements, please contact us to determine which Epinn model is appropriate for your sample type.

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