Cyclomics
CySeq® Amplicon Solution
The CySeq® Amplicon Solution brings single-molecule consensus accuracy to any targeted PCR panel. Each amplicon, whether from a Cyclomics®-designed panel or a custom in-house assay, is crosslinked, concatemerized and sequenced before variant reporting. The output gives you the confidence to act on low frequency results—without UMIs, and without workflow redesign.
At a Glance
✓ Confident variant calls >0.5% VAF
✓ Q40+ sequencing accuracy on ONT platforms without UMIs
✓ Compatible with existing PCR panel
✓ Single-molecule high accuracy
✓ PCR to result in ~6 hours
✓ Compatible with ONT sequencing or any other long-read sequencing technology
✓ Panel performance metrics
The Solution
Tumour-derived variants can be present at allele frequencies well below the noise floor of standard sequencing, discarding a significant portion of the available biological signal.
The CySeq® cfDNA Amplicon Solution addresses this by applying consensus error-correction at the single-molecule level before sequencing ever begins. The result is high accuracy variant calling on consensus reads with Q40+ accuracy on Oxford Nanopore platforms.
Step by Step
01
PCR amplification
Generate amplicons (50–500 bp) using either a ready-made Cyclomics® panel or your own custom in-house PCR panel.
02
CySeq short fragment sequencing kit
Concatemerize each PCR amplicon molecule and amplify head-to-tail into tandem molecular repeats. This is the core proprietary step.
03
Nanopore sequencing
Sequence concatemers on any long-read sequencer. Multiple repeat reads per molecule feed the consensus algorithm.
04
Consensus and variant calling
Algorithm aligns repeats per molecule, cancels random errors, and outputs high-confidence variant calls down to 0.5% VAF