Cyclomics
CySeq® ALK-TRACK
Targeted and highly accurate ALK monitoring in circulating cell-free DNA from 0.5 - 1 ml blood draw. All clinically relevant ALK variants in a single assay, with turnaround time from sample to result in 4 days.
Key Highlights
✓ Targeted: 6 amplicons covering 19 clinically relevant ALK sites
✓ Sensitive: Designed for low-frequency variant detection
✓ Minimally invasive: Analysis from approximately 200 µL of blood
✓ Longitudinal: Enables repeated monitoring of ALK mutations
✓ Rapid: Approximately 4 days to 1-week turnaround time
The Workflow
Step by Step
After cfDNA isolation, the DNA is amplified using the ALK-TRACK Multiplex PCR Panel (Step 1) to enrich the clinically relevant regions of the ALK kinase domain. The resulting PCR products are then processed with the CySeq® Short Fragment Sequencing solution (Step 2), where DNA molecules are circularized and amplified by rolling circle amplification (RCA) to generate long concatemers containing multiple copies of the original molecules. The concatemers are prepared for Oxford Nanopore sequencing (Step 3), enabling consensus-based analysis for sensitive detection of low-frequency ALK mutations in liquid biopsy samples (Step 4).