Cyclomics
CySeq® cfDNA Whole Genome Solution
Our CySeq® cfDNA Whole Genome Solution offers a consensus sequencing DNA preparation kit with dedicated analysis software. The solution delivers genome-wide insight from both single-stranded and double-stranded cell-free DNA — producing high quality consensus reads with calibrated Q scores and Q40+ sequencing accuracy on ONT platforms. By resolving ssDNA and dsDNA fragment size distributions and providing annotated dsDNA overhangs, it enables the investigation of fragmentomics and strand-specific mutagenesis at high accuracy.
At a Glance
✓ High quality consensus reads with calibrated Q scores
✓ Q40+ sequencing accuracy on ONT platforms without UMIs
✓ ssDNA and dsDNA resolved fragment size distribution
✓ Annotated dsDNA overhangs
✓ One-pot workflow, streamlines handling and cuts hands-on time
✓ Sample to result in ~6 hours
✓ Compatible with ONT sequencing or any other long-read sequencing technology
The Solution
Cell-free DNA circulating in plasma carries important information about cancer: tumour-derived mutations, copy number alterations, fragmentation signatures, and strand-specific damage profiles, all accessible from a simple blood draw. The challenge is extracting that signal reliably.
The CySeq® cfDNA Whole Genome Solution addresses this by applying consensus error-correction at the single-molecule level before sequencing ever begins. The result is comprehensive, genome-wide cfDNA profiling with Q40+ accuracy on Oxford Nanopore sequencing platforms — capturing mutations, fragmentomics and DNA end structures in a single workflow.
Four integrated steps take a raw plasma sample to a consensus-called variant report. The CySeq® Short Fragment sequencing kit drives Steps 2–4.
Step by Step
01
cfDNA extraction
Isolate cell-free DNA from raw plasma with high recovery. Both ssDNA and dsDNA are captured without end modification.
02
CySeq short fragment sequencing kit
Concatemerize each cfDNA molecule and amplify head-to-tail into tandem molecular repeats. This is the core proprietary step.
03
Nanopore sequencing
Sequence concatemers on any long-read sequencer. Multiple repeat reads per molecule feed the consensus algorithm.
04
Consensus and variant calling
Algorithm aligns repeats per molecule, cancels random errors, and outputs high-confidence variant calls.